Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 HDPCs and cultured for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Suspension, Cell Culture

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Singularized HDPC response to culture in egg crate-designed microwell arrays fabricated from different non-adherent substrate materials. Stereolithography 3D printed stamps with an egg crate microwell pattern were used to mold 2% agarose ( A ), 5% agarose ( B ), and PDMS ( C ) substrate materials. HDPCs at 3000 cells per microwell were added to the arrays, cultured for 24 h, and imaged using light microscopy. The softer 2% and 5% agarose hydrogel substrate materials did not result in spheroid formation but did effectively pattern the singularized HDPCs. The stiffer silicone elastomer PDMS microwell arrays did result in HDPC spheroid formation and patterning when seeded with singularized HDPCs. Scale bars = 200 µm.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Cell Culture, Light Microscopy

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Distance between HDPC spheroids over time. Quantification of the distance of patterned HDPC spheroids formed in PDMS microwell arrays (1 d), transferred to the collagen matrix dermal compartment (3 d), cultured in epidermalization media (5 d), and transitioned to the air–liquid interface. The average distance of patterned spheroids decreases as the collagen matrix contracts and is reorganized by HDPCs. Scale bars = 200 µm. Note that the 1 d image on the top left is the same image shown in C. Significance comparing microwell spheroid distance at 1 day to later timepoints is denoted at statistical levels: *** p < 0.001.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Cell Culture

Journal: Frontiers in Pharmacology

Article Title: The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

doi: 10.3389/fphar.2025.1570922

Figure Lengend Snippet: G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

Article Snippet: Human hair follicle dermal papilla cells (hDPCs) were obtained from PromoCell (Heidelberg, Germany) and cultured in follicle dermal papilla cell growth medium (Promocell) supplemented with a growth medium Supplement Pack (Promocell).

Techniques: Expressing, Quantitative RT-PCR, Activation Assay, Western Blot